Alexandra R. Fernandes

Professor Auxilar

303
Lab 319

11107
ma.fernandes@fct.unl.pt

Áreas de Investigação

Cancer mechanisms and Therapy - novel compounds with anti-cancer activity and their potential use in therapy.
Cancer Diagnostics [Co-Founder of FCT/UNL Start up - Nano4 Global, Lda, Nano4Global.com]
Cardiology - molecular mechanisms of cardiomyopathies with particular empahsis in Hypertrophic cardiomyopathy (HCM) molecular profiling - [co-Founder of HeartGenetics, SA - Molecular diagnostics Start up - Heartgenetics.com ]

Publicações Representativas

2017


Rita Mendes, Pedro Pedrosa, João C. Lima, Alexandra R. Fernandes*, Pedro V. Baptista*. Photothermal enhancement of chemotherapy in breast cancer by visible irradiation of Gold Nanoparticles. Scientific Reports 2017 (accepted)


Kuheli Das, Belete B. Beyene, Amitabha Datta,* Catarina Roma-Rodrigues, Mariana Barroso, Pedro V. Baptista, Alexandra R. Fernandes,* Chen-Hsiung Hung,* A Schiff-base Assisted Cu(II) Derivative: Electrocatalytic H2 Evolution and Biological studies on human cancer cell lines. JBIC  2017 (minor revisions).


Gomes S.E., Pereira D.M., Roma-Rodrigues C., Fernandes A.R.*, Borralho P.M.B., Rodrigues C.M.P.*. Proteomic analysis of HCT116 colon cancer cells overexpressing miR-143 and miR-145. (submitted).


Joana R. Sousa, Célia M. Silveira, Catarina Roma-Rodrigues, Alexandra R. Fernandes, Bart Devreese, Isabel Moura, José J.G. Moura, M. Gabriela Almeida,*. Understanding the response of Desulfovibrio desulfuricans ATCC 27774 to different electron acceptors - biosynthetic costs modulate substrate selection. BBA - Proteins and Proteomics 2017 (accepted)


Luiz Gonzaga do Nascimento-Netoa, Maria Guadalupe Cabral, Rômulo Farias Carneiro, Francisco Vassiliepe Sousa Arruda, Zélia Silva, Celso Shiniti Nagano, Alexandra R Fernandes, Alexandre Holanda Sampaio, Edson Holanda Teixeira and Paula Alexandra Videira. Halilectin-3, a lectin from the marine sponge Haliclona caerulea, induces apoptosis and autophagy in human breast cancer MCF7 cells through a caspase-9 and LC3 pathway. Anti-Cancer Agents in Medicinal Chemistry 2017 (accepted).


Katarzyna Czerwinska, Barbara Machura* Slawomir Kula, Stanislaw Krompiec, Catarina Roma-Rodrigues, Alexandra R. Fernandes*, Lidia S. Shul’pina and Georgiy B. Shul’pin*. Copper(II) complexes of functionalized 2,2':6',2''-terpyridines and 2,6-di(thiazol-2-yl)pyridine: structure, spectroscopy, cytotoxicity and catalytic activity. Dalton Trans. 2017 (accepted).


Manas Sutradhar,* Rajeshwari, Tannistha Roy Barman, Alexandra R Fernandes* Fabiana Paradinha, Catarina Roma-Rodrigues, M. Fátima C. Guedes da Silva, and Armando J. L. Pombeiro*. Mixed ligand aroylhydrazone and N-donor heterocyclic Lewis base Cu(II) complexes as potential antiproliferative agents. J Inorg Biochem 2017 (accepted).


Márcia Tavares, Renato P. Cabral, Clarinda Costa, Pedro Martins, Alexandra R. Fernandes, Teresa Casimiro, Ana Aguiar-Ricardo. Development of PLGA dry powder microparticles by supercritical CO2-assisted spray-drying for potential vaccine delivery to the lungs. The Journal of Supercritical Fluids 2017; 128: 235-243.


Oscar A. Lenis-Rojas, Catarina Roma-Rodrigues, Alexandra R. Fernandes*, Fernanda Marques, David Pérez-Fernández, Jorge Guerra-Varela, Laura Sánchez, Digna Vázquez-García, Margarita López Torres, Alberto Fernández, Jesús J. Fernández*. Dinuclear RuII(bipy)2 Derivatives: Structural, Biological and In Vivo Zebrafish Toxicity Evaluation. Inorganic Chemistry 2017. 56 (12), pp 7127–7144.


Alexandra R. Fernandes*, João Jesus, Pedro Martins, Sara Figueiredo, Daniela Rosa, Luísa M.R.D.R.S. Martins, Maria Luísa Corvo, Manuela C. Carvalheiro, Pedro M. Costa, Pedro V. Baptista*. Multifunctional gold-nanoparticles: a nanovectorization tool for the targeted delivery of novel chemotherapeutic agents. Journal of Control Release 2017, 245:52-61. doi.org/10.1016/j.jconrel.2016.11.021.


Raquel Vinhas, Alexandra R. Fernandes*, Pedro V. Baptista*. Gold nanoparticles for BCR-ABL1 gene silencing – Improving tyrosine kinase inhibitors efficacy in chronic myeloid leukemia. Mol Ther Nucleic Acids. 2017, 7:408-416. doi.org/10.1016/j.omtn.2017.05.003.


Rita Mendes, Alexandra R. Fernandes* and Pedro V. Baptista*. Gold nanoparticle approach to the selective delivery of gene silencing in cancer – the case for combined delivery? Clinical Genomics in Genetic Diseases and Cancer. Genes 2017, 8:94.


Catarina Roma-Rodrigues, Francisca Pereira, António P. Alves de Matos, Marta Fernandes, Pedro V. Baptista,* AND Alexandra R. Fernandes,* Smuggling gold nanoparticles across cell types – a new role for exosomes in gene silencing. Nanomedicine. 2017 Jan 28;13(4):1389-1398.


Catarina Roma-Rodrigues, Luís R. Raposo, Rita Cabral, Fabiana Paradinha, Pedro V Baptista* AND Alexandra R Fernandes*. Tumor microenvironment modulation via gold nanoparticles targeting malicious exosomes: implications in cancer diagnostics and therapy. Int J Mol Sci. 2017 Jan 14;18(1).


Luís R. Raposo, Catarina Roma-Rodrigues, João Jesus, Luísa M.D.R.S. Martins, Armando J Pombeiro, Pedro V. Baptista*, Alexandra R. Fernandes*. Targeting canine mammary tumors via gold nanoparticles functionalized with promising Co(II) and Zn(II) compounds. Veterinary and Comparative Oncology 2017; 1–6.


Milton Cordeiro, Lara Carvalho, Joana Filipa Silva, Leonor Saúde, Alexandra R Fernandes, Pedro V. Baptista *. Gold nanobeacons for tracking gene silencing in zebrafish. Nanomaterials (Basel). 2017 Jan 11;7(1).


2016


Susana I. C. J. Palma, Alexandra R. Fernandes and Ana C. A. Roque. An affinity triggered MRI nanoprobe for pH-dependent cell labeling. RSC Adv., 2016,6, 113503-113512.


Raquel Vinhas, Milton Cordeiro, Pedro Pedrosa, Alexandra R. Fernandes*, Pedro V. Baptista*. Current trends in molecular diagnostics of Chronic Myeloid Leukemia. Leuk Lymphoma. 2017,58:1791-1804


O. A. Lenis-Rojas, A.R. Fernandes*, C. Rodrigues, P. V. Baptista, F. M. Marques, D. Pérez-Fernández, J. Guerra, L. E. Sanchez, D. Vazquez-Garcia, M. Lopez-Torres, A. Fernández and J. J. Fernández Sánchez*. Heteroleptic mononuclear compounds of ruthenium(II): synthesis, structural analyses, in vitro antitumor activity and in vivo toxicity on zebrafish embryos. Dalton Trans., 2016,45:19127-19140.


Mariana Silva, Zélia Silva, Graça Marques, Tiago Ferro, Márcia Gonçalves, Mauro Monteiro, Sandra J. van Vliet, Elodie Mohr, Andreia C. Lino, Alexandra R. Fernandes, Flávia A. Lima, Yvette van Kooyk, Teresa Matos, Carlos E. Tadokoro and Paula A. Videira. Sialic acid removal from dendritic cells improves antigen cross-presentation and boosts anti-tumor immune responses. Oncotarget 2016, 7:41053-41066.


Raquel Vinhas, Cláudia Correia, Patricia Ribeiro, Alexandra Lourenço, Aida Botelho de Sousa, Alexandra R. Fernandes AND Pedro V. Baptista*. Colorimetric assessment of BCR-ABL1 transcripts in clinical samples via gold nanoprobes. Anal Bioanal Chem 2016, 408:5277–5284.


C. Roma-Rodrigues, A. Heuer-Jungemann, A. R. Fernandes, A. G. Kanaras and P. V. Baptista. Peptide coated gold nanoparticles for in vivo targeting of angiogenesis. Int J Nanomedicine. 2016; 11: 2633–2639.


Rita Mendes, Bárbara Carreira, Pedro V. Baptista* and Alexandra R. Fernandes*. Non-small lung cancer biomarkers and targeted therapy - two faces of the same coin fostered by Nanotechnology. Expert Review of Precision Medicine and Drug Development. 2016; 1(2): 155-168.


Luisa Corvo, Ana Soraia Mendo, Sara Figueiredo, Miguel Larguinho, Rogério Gaspar, Pedro V. Baptista* and Alexandra R. Fernandes*. Liposomes as delivery system of a Sn(IV) compound for cancer therapy. Pharm Res. 2016 Jun;33(6):1351-8. doi: 10.1007/s11095-016-1876-6.


Luís R. Raposo, Catarina Roma-Rodrigues, Pedro Faísca, Margarida Alves, Joaquim Henriques, Manuela C. Carvalheiro, M. Luisa Corvo, Pedro V. Baptista, Armando JL Pombeiro, Alexandra R. Fernandes*. Immortalization and characterization of a new canine mammary tumor cell line FR37-CMT. J. Veterinary and Comparative Oncology 2016. DOI: 10.1111/vco.12235.


Marta Martins, Pedro V. Baptista, Ana Soraia Mendo, Claudia Correia, Paula Videira, António S. Rodrigues, J. Muthukumaran, Teresa Santos-Silva, Ana Silva, M. Fátima C. Guedes da Silva, Joana Gigante, António Duarte, Armando J. L. Pombeiro, Alexandra R. Fernandes*. In vitro and in vivo biological characterization of the anti-proliferative potential of a cyclic trinuclear organotin(IV) complex.  Mol Biosyst. 2016 Mar;12(3):1015-23. doi: 10.1039/c5mb00791g.


Zhen Ma*, Bian Zhang, M. Fátima C. Guedes da Silva, Ana Soraia Mendo, Joana Silva, Pedro V. Baptista, Alexandra R. Fernandes*, Armando J.L. Pombeiro*. Synthesis, Characterization, Thermal Properties and Antiproliferative Potential of Copper(II) 4′-phenyl-terpyridine Compounds. Dalton Trans. 2016 Mar 28;45(12):5339-55. doi: 10.1039/c5dt02744f.


Sutradhar, M, Fernandes AR*, Silva J, Mahmudov KT, da Silva FGMC, Pombeiro AJL.  Water soluble heterometallic potassium-dioxidovanadium(V) (K+/VO3+) complexes as potential antiproliferative agents. J Inorg Biochem. 2016; 155:17-25.


Catarina Roma-Rodrigues, Cynthia Barroco, Luís R Raposo, Mafalda N Costa, Elvira Fortunato, Pedro Viana Baptista, Alexandra R Fernandes*, Ilda Santos-Sanches*. Infection of human keratinocytes by Streptococcus dysgalactiae subspecies dysgalactiae isolated from milk of the bovine udder. Microbes Infect. 2016 Apr;18(4):290-3. doi: 10.1016/j.micinf.2015.11.005.


Raquel Vinhas, Anna Tolmatcheva, Rafaela Canto, Patricia Ribeiro, Alexandra Lourenço, Aida Botelho de Sousa, Pedro V. Baptista, Alexandra R. Fernandes*. A novel mutation in the CEBPA gene in a patient with acute myeloid leukemia. Leuk Lymphoma. 2016;57(3):711-3. doi: 10.3109/10428194.2015.


2015


Pedro Martins, João Jesus, Sofia Santos, Luis R. Raposo, Catarina Roma-Rodrigues, Pedro Viana Baptista, and Alexandra R. Fernandes*. Heterocyclic Anticancer Compounds: Recent Advances and the Paradigm Shift towards the Use of Nanomedicine’s Tool Box. Molecules 2015, 20(9), 16852-16891; doi:10.3390/molecules200916852.


Susana I. C. J. Palma. Carlos A. V. Rodrigues, Filomena Freitas, Alexandra Carvalho, Alexandra R. Fernandes, Maria del Puerto Morales, Joaquim M.S. Cabral, Ana C. A. Roque. A value-added exopolysaccharide as a coating agent for MRI nanoprobes. Nanoscale. 2015 Sep 14;7(34):14272-83. doi: 10.1039/c5nr01979f.


Ana Soraia Mendo, Sara Figueiredo, Catarina Roma-Rodrigues, Paula A. Videira, Zhen Ma, Mário Diniz, Miguel Larguinho, Pedro M. Costa, Armando J.L. Pombeiro, Pedro V. Baptista, Alexandra R. Fernandes*. Characterization of antiproliferative potential and biological targets of a copper complex containing 4’-phenyl terpyridine. J Biol Inorg Chem. 2015 Sep;20(6):935-48. doi: 10.1007/s00775-015-1277-z.


R.B. Restani, J. Conde, R.F. Pires, P. Martins, A.R. Fernandes, P.V. Baptista, V.D.B. Bonifácio, A. Aguiar-Ricardo. POxylated polyurea dendrimers: Smart core-shell vectors with IC50 lowering capacity. Macromol Biosci. 2015 Aug;15(8):1045-51. doi: 10.1002/mabi.201500131.


Raquel Vinhas, Milton Cordeiro, Fábio Ferreira Carlos, Soraia Mendo, Alexandra R. Fernandes, Sara Figueiredo, Pedro V. Baptista. Gold nanoparticle-based theranostics: disease diagnostic and treatment using a single nanomaterial. J. Nanobiosensors in Disease Diagnosis 2015; 4:11–23


Susana I.C.J.Palma, Alexandra Carvalho, Joana Silva, Alexandra R Fernandes, Maria del Puerto-Morales, Ana C. A. Roque. Covalent coupling of gum arabic onto superparamagnetic iron oxide nanoparticles for MRI cell labeling: physiochemical and in vitro characterization. Contrast Media Mol Imaging. 2015 Jul-Aug;10(4):320-8. doi: 10.1002/cmmi.1635.


Catarina Roma-Rodrigues, Luís R. Raposo, Alexandra R. Fernandes*. microRNAs based therapy of Hypertrophic cardiomyopathy: the road traveled so far. BioMed Research International 2015; 2015, Article ID 983290, 8 pages.http://dx.doi.org/10.1155/2015/983290.


Coimbra J, Mota C, Santos S, Baptista PV, Fernandes AR*. Inorganic Compounds Going NANO. Ann Med Chem Res 2015; 1(2): 1010


Pedro Pedrosa, Raquel Vinhas, Alexandra R Fernandes, Pedro V Baptista. Gold nanotheranostics: proof-of-concept or clinical tool? Nanomaterials 2015; 5(4): 1853-79.


 2014


Pedro Martins, Mara Marques, Lídia Coito, Armando J.L. Pombeiro, Pedro Viana Baptista and Alexandra R Fernandes*. Organometallic Compounds in Cancer Therapy: Past Lessons and Future Directions. Anti-Cancer Agents in Medicinal Chemistry 2014; 14(9):1199-1212. DOI: 10.2174/1871520614666140829124925


Joana Silva, António Sebastião Rodrigues, Paula A. Videira, Jamal Lasri, Adília Januário Charmier, Armando J.L. Pombeiro, and Alexandra R. Fernandes*. Characterization of the antiproliferative potential and biological targets of a trans ketoimine platinum complex. Inorganica Chimica Acta 2014; 423:156-167. DOI: 10.1016/j.ica.2014.07.067


Catarina Roma-Rodrigues, Alexandra R. Fernandes*. The Genetics of Hypertrophic Cardiomyopathy: advances and pitfalls in molecular diagnosis and therapy. The Application of Clinical Genetics 2014; 7: 195–208.


Catarina Roma-Rodrigues, Alexandra R. Fernandes* and Pedro V. Baptista*. Exosome in Tumour Microenvironment: Overview of the Crosstalk between Normal and Cancer Cells. BioMed Research International 2014; 2014, 10 pages. http://dx.doi.org/10.1155/2014/179486.


Pedro Martins, Daniela Rosa, Alexandra R. Fernandes and Pedro V. Baptista. Nanoparticle Drug Delivery Systems: Recent Patents and Applications in Nanomedicine. Recent Patents on Nanomedicine 2014; 3: 105-118. DOI: 10.2174/1877912304666140304000133.


João Conde, Miguel Larguinho, Ana Cordeiro, Luís R. Raposo, Pedro M. Costa, Susana Santos, Mário S. Diniz, Alexandra R. Fernandes, Pedro V. Baptista. Gold-nanobeacons for gene therapy: evaluation of genotoxicity, cell toxicity and proteome profiling analysis. Nanotoxicology 2014; 8: 521-532. DOI:10.3109/17435390.2013.802821.


Kamran T. Mahmudov, M. Fátima C. Guedes da Silva, Archana Mizar, Maximilian N. Kopylovich, Alexandra R. Fernandes*, Ana Silva, Armando J. L. Pombeiro. Di- and triorganotin(IV) complexes of arylhydrazones of methylene active compounds and their antiproliferative activity. J. Organometellic Chemistry 2014; 760: 67–73. (DOI: 10.1016/j.jorganchem.2013.12.019)


Daniel V. Luís, Joana Silva, Ana Isabel Tomaz, Rodrigo F.M. de Almeida, M. Larguinho, PV Baptista, Luísa M.D.R.S. Martins, Telma F.S. Silva, Pedro M. Borralho, Cecília M.P. Rodrigues, António S. Rodrigues, Armando J.L. Pombeiro, Alexandra R. Fernandes*. Insights into the mechanisms underlying the anti-proliferative potential of a Co(II) coordination compound bearing 1,10-phenanthroline-5,6-dione: DNA and protein interaction studies. J Biol Inorg Chem. 2014; 19: 787-803. DOI 10.1007/s00775-014-1110-0.


Telma F.S. Silva, Luísa M.D.R.S. Martins, M. Fátima Guedes da Silva, Maxim L. Kuznetsov, Alexandra R. Fernandes, Ana Silva, Chun-Jern Pan, Jyh-Fu Lee, Bing-Joe Hwang, and Armando J.L. Pombeiro. Cobalt complexes bearing scorpionate or pyrazole ligands as catalysts for the peroxidative oxidation of cyclohexane. XAS studies and biological applications. Chemistry – an Asian Journal 2014; 9, 1132-1143  http://dx.doi.org/10.1002/asia.201301331.